THERMOSPRAY LIQUID-CHROMATOGRAPHIC MASS-SPECTROMETRIC ANALYSIS OF ANTI-AIDS NUCLEOSIDES - QUANTIFICATION OF 2',3'-DIDEOXYCYTIDINE IN PLASMA SAMPLES

被引：17

作者：

JAJOO, HK

BENNETT, SM

KORNHAUSER, DM

机构：

[1] JOHNS HOPKINS UNIV,SCH MED,DEPT MED,DIV CLIN PHARMACOL,BALTIMORE,MD 21205

[2] JOHNS HOPKINS UNIV,SCH MED,DEPT PHARMACOL & MOLEC SCI,BALTIMORE,MD 21205

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 577卷 / 02期

关键词：

D O I：

10.1016/0378-4347(92)80251-K

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Thermospray liquid chromatography-mass spectrometry was investigated as a method for quantification of 2',3'-dideoxycytidine (DDC) from human plasma. A stable isotope analog of DDC ([N-15(2),H-2(2)]DDC) was used as an internal standard. Selected ion monitoring of the protonated molecular ions for DDC and the internal standard was used to record mass chromatograms. The areas of the peaks in the mass chromatograms were used for quantification. The detection limit of DDC in this assay was 50 pg on-column. The calibration curve was linear over the desired range. 0.25-20 ng/ml. The major advantages of this assay over others are: no derivatization. high sensitivity, high specificity and short assay time.

引用

收藏

页码：299 / 304

页数：6

相关论文

共 18 条

[1] ISOLATION OF A T-LYMPHOTROPIC RETROVIRUS FROM A PATIENT AT RISK FOR ACQUIRED IMMUNE-DEFICIENCY SYNDROME (AIDS) [J].

BARRESINOUSSI, F ;

CHERMANN, JC ;

REY, F ;

NUGEYRE, MT ;

CHAMARET, S ;

GRUEST, J ;

DAUGUET, C ;

AXLERBLIN, C ;

VEZINETBRUN, F ;

ROUZIOUX, C ;

ROZENBAUM, W ;

MONTAGNIER, L .

SCIENCE, 1983, 220 (4599) :868-871

[2] APPLICATION OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND THERMOSPRAY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY TO THE ANALYSIS AND IDENTIFICATION OF 2',3'-DIDEOXYADENOSINE AND ITS METABOLITE IN BIOLOGICAL MEDIA [J].

BLAU, PA ;

HINES, JW ;

VOYKSNER, RD .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1987, 420 (01) :1-12

[3] A PATHOGENIC RETROVIRUS (HTLV-III) LINKED TO AIDS [J].

BRODER, S ;

GALLO, RC .

NEW ENGLAND JOURNAL OF MEDICINE, 1984, 311 (20) :1292-1297

[4] THERMOSPRAY LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY OF NUCLEOSIDES AND OF ENZYMATIC HYDROLYSATES OF NUCLEIC-ACIDS [J].

EDMONDS, CG ;

VESTAL, ML ;

MCCLOSKEY, JA .

NUCLEIC ACIDS RESEARCH, 1985, 13 (22) :8197-8206

[5] DIRECT LIQUID INTRODUCTION LC MS MICROBORE EXPERIMENTS FOR THE ANALYSIS OF NUCLEOSIDE MATERIAL PRESENT IN HUMAN-URINE [J].

ESMANS, EL ;

GEBOES, P ;

LUYTEN, Y ;

ALDERWEIRELDT, FC .

BIOMEDICAL MASS SPECTROMETRY, 1985, 12 (05) :241-245

[6] ANALYSIS OF SUBSTITUTED PYRIDINE-C-NUCLEOSIDES BY DIRECT LIQUID INTRODUCTION LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY [J].

ESMANS, EL ;

BELMANS, M ;

VRIJENS, I ;

LUYTEN, Y ;

ALDERWEIRELDT, FC ;

WOTRING, LL ;

TOWNSEND, LB .

NUCLEOSIDES & NUCLEOTIDES, 1987, 6 (05) :865-876

[7] THE EFFICACY OF AZIDOTHYMIDINE (AZT) IN THE TREATMENT OF PATIENTS WITH AIDS AND AIDS-RELATED COMPLEX - A DOUBLE-BLIND, PLACEBO-CONTROLLED TRIAL [J].

FISCHL, MA ;

RICHMAN, DD ;

GRIECO, MH ;

GOTTLIEB, MS ;

VOLBERDING, PA ;

LASKIN, OL ;

LEEDOM, JM ;

GROOPMAN, JE ;

MILDVAN, D ;

SCHOOLEY, RT ;

JACKSON, GG ;

DURACK, DT ;

KING, D .

NEW ENGLAND JOURNAL OF MEDICINE, 1987, 317 (04) :185-191

[8]

GUSTAVSON LE, 1990, J ACQ IMMUN DEF SYND, V3, P28

[9]

HAO Z, 1988, MOL PHARMACOL, V34, P431

[10] PHARMACOKINETICS OF 2',3'-DIDEOXYCYTIDINE IN PATIENTS WITH AIDS AND RELATED DISORDERS [J].

KLECKER, RW ;

COLLINS, JM ;

YARCHOAN, RC ;

THOMAS, R ;

MCATEE, N ;

BRODER, S ;

MYERS, CE .

JOURNAL OF CLINICAL PHARMACOLOGY, 1988, 28 (09) :837-842