SELF REACTIVE REPERTOIRE OF TIGHT SKIN MOUSE - IMMUNOCHEMICAL AND MOLECULAR CHARACTERIZATION OF ANTI-TOPOISOMERASE-I AUTOANTIBODIES

被引：32

作者：

MURYOI, T ^{[1
]}

KASTURI, KN ^{[1
]}

KAFINA, MJ ^{[1
]}

SAITOH, Y ^{[1
]}

USUBA, O ^{[1
]}

PERLISH, JS ^{[1
]}

FLEISCHMAJER, R ^{[1
]}

BONA, CA ^{[1
]}

机构：

[1] CUNY MT SINAI SCH MED,DEPT MICROBIOL,BOX 1124,1 GUSTAVE L LEVY PL,NEW YORK,NY 10029

来源：

AUTOIMMUNITY | 1991年 / 9卷 / 02期

关键词：

TIGHT SKIN MOUSE; ANTI-TOPOISOMERASE-I AUTOANTIBODIES; CROSS REACTIVE IDIOTYPE; V-GENE USAGE; PROGRESSIVE SYSTEMIC SCLEROSIS;

D O I：

10.3109/08916939109006746

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Tight skin (TSK) mice develop cutaneous hyperplasia accompanied by histopathological alterations of skin and collagen metabolism similar to those described in human scleroderma. Diffuse scleroderma, the most severe form of progressive systemic sclerosis, is associated with the production of autoantibodies specific for Scleroderma 70 antigen (topoisomerase I). Our studies show that there is an increase in the level of serum anti-topoisomerase I (topo I) autoantibodies in aged TSK mice. The monoclonal antibodies isolated from TSK mice bind to epitopes which interact with autoantibodies from scleroderma patients. A significant number of TSK monoclonal anti-topo I antibodies and serum immunoglobulin (Ig) from aged TSK mice bear a cross reactive idiotype (Id) recognized by a syngeneic monoclonal anti-Id antibody obtained from a 2 month-old TSK mouse. Analysis of V gene usage by monoclonal anti-topo I antibodies showed that the majority of these antibodies are encoded by VH genes derived from VH 5558 family pairing with VK, genes from various families in a stochastic manner. © 1991 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：109 / 117

页数：9

共 28 条

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