ISOLATION AND CHARACTERIZATION OF CDNAS ENCODING WHEAT 3-HYDROXY-3-METHYLGLUTARYL COENZYME-A REDUCTASE

被引：43

作者：

AOYAGI, K ^{[1
]}

BEYOU, A ^{[1
]}

MOON, K ^{[1
]}

FANG, L ^{[1
]}

ULRICH, T ^{[1
]}

机构：

[1] SOGETAL INC,HAYWARD,CA 94545

来源：

PLANT PHYSIOLOGY | 1993年 / 102卷 / 02期

关键词：

D O I：

10.1104/pp.102.2.623

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR, EC 1.1.1.34) is a key enzyme in the isoprenoid biosynthetic pathway. We have isolated partial cDNAs from wheat (Triticum aestivum) using the polymerase chain reaction. Comparison of deduced amino acid sequences of these cDNAs shows that they represent a small family of genes that share a high degree of sequence homology among themselves as well as among genes from other organisms including tomato, Arabidopsis, hamster, human, Drosophila, and yeast. Southern blot analysis reveals the presence of at least four genes. Our results concerning the tissue-specific expression as well as developmental regulation of these HMGR cDNAs highlight the important role of this enzyme in the growth and development of wheat.

引用

页码：623 / 628

页数：6

共 27 条

[1] AOYAGI K, 1991, J CELL BIOCH SUPPL A, V15, P142
[2] STRUCTURAL AND FUNCTIONAL CONSERVATION BETWEEN YEAST AND HUMAN 3-HYDROXY-3-METHYLGLUTARYL COENZYME-A REDUCTASES, THE RATE-LIMITING ENZYME OF STEROL BIOSYNTHESIS
BASSON, ME
THORSNESS, M
FINERMOORE, J
STROUD, RM
RINE, J
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (09) : 3797 - 3808
[3] STRUCTURAL-ANALYSIS OF NUCLEAR GENES-CODING FOR THE PRECURSOR TO THE SMALL SUBUNIT OF WHEAT RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE
BROGLIE, R
CORUZZI, G
LAMPPA, G
KEITH, B
CHUA, NH
[J]. BIO-TECHNOLOGY, 1983, 1 (01): : 55 - 61
[4] SUBCELLULAR-LOCALIZATION OF 3-HYDROXY-3-METHYLGLUTARYL COENZYME A REDUCTASE IN PISUM-SATIVUM SEEDLINGS
BROOKER, JD
RUSSELL, DW
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1975, 167 (02) : 730 - 737
[5] BIOGENESIS OF 3-HYDROXY-3-METHYLGLUTARYL-COENZYME-A REDUCTASE, AN INTEGRAL GLYCOPROTEIN OF THE ENDOPLASMIC-RETICULUM
BROWN, DA
SIMONI, RD
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (06): : 1674 - 1678
[6] ISOLATION AND STRUCTURAL CHARACTERIZATION OF A CDNA-ENCODING ARABIDOPSIS-THALIANA 3-HYDROXY-3-METHYLGLUTARYL COENZYME-A REDUCTASE
CAELLES, C
FERRER, A
BALCELLS, L
HEGARDT, FG
BORONAT, A
[J]. PLANT MOLECULAR BIOLOGY, 1989, 13 (06) : 627 - 638
[7] NUCLEOTIDE-SEQUENCE OF 3-HYDROXY-3-METHYL-GLUTARYL COENZYME-A REDUCTASE, A GLYCOPROTEIN OF ENDOPLASMIC-RETICULUM
CHIN, DJ
GIL, G
RUSSELL, DW
LISCUM, L
LUSKEY, KL
BASU, SK
OKAYAMA, H
BERG, P
GOLDSTEIN, JL
BROWN, MS
[J]. NATURE, 1984, 308 (5960) : 613 - 617
[8] ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE
CHIRGWIN, JM
PRZYBYLA, AE
MACDONALD, RJ
RUTTER, WJ
[J]. BIOCHEMISTRY, 1979, 18 (24) : 5294 - 5299
[9] CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[10] CHARACTERIZATION OF CDNA AND GENOMIC CLONES ENCODING 3-HYDROXY-3-METHYLGLUTARYL-COENZYME-A REDUCTASE FROM HEVEA-BRASILIENSIS
CHYE, ML
KUSH, A
TAN, CT
CHUA, NH
[J]. PLANT MOLECULAR BIOLOGY, 1991, 16 (04) : 567 - 577

← 1 2 3 →