GAMMA-IRRADIATION INCREASES HSP-70 IN CHINESE-HAMSTER OVARY CELLS

被引：60

作者：

SIERRARIVERA, E ^{[1
]}

VOORHEES, GJ ^{[1
]}

FREEMAN, ML ^{[1
]}

机构：

[1] VANDERBILT UNIV,MED CTR,SCH MED,VANDERBILT CTR RADIAT ONCOL,B-902 TVC,NASHVILLE,TN 37232

来源：

RADIATION RESEARCH | 1993年 / 135卷 / 01期

关键词：

D O I：

10.2307/3578394

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The induction of hsp-70 by γ irradiation was studied in Chinese hamster ovary (CHO) cells. Synthesis of hsp-70 was assessed by SDS-PAGE of radiolabeled proteins while levels of hsp-70 mRNA were estimated by Northern blot analysis of total RNA. SDS-PAGE analysis indicated that the synthesis of hsp-70 was enhanced in cells exposed to 400 or 1000 Gy. Northern blot analysis of RNA from cells exposed to 1000 Gy indicated a rapid increase in mRNA capable of hybridizing to an hsp-70 oligonucleotide probe. These experiments represent the first report demonstrating that ionizing radiation can increase hsp-70 mRNA.

引用

页码：40 / 45

页数：6

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