URINARY-EXCRETION OF PYRIDINOLINES CROSS-LINKS MEASURED BY IMMUNOASSAY AND HPLC TECHNIQUES IN NORMAL SUBJECTS AND IN ELDERLY PATIENTS WITH VITAMIN-D DEFICIENCY

被引:17
作者
KAMEL, S
BRAZIER, M
PICARD, C
BOITTE, F
SAMSON, L
DESMET, G
SEBERT, JL
机构
[1] CHU AMIENS,HORMONOL LAB,F-80054 AMIENS 01,FRANCE
[2] CHU AMIENS,SERV RHUMATOL,F-80054 AMIENS 01,FRANCE
[3] BEHRING DIAGNOST,F-92504 RUEIL MALMAISON,FRANCE
来源
BONE AND MINERAL | 1994年 / 26卷 / 03期
关键词
PYRIDINOLINES; URINE; SECONDARY HYPERPARATHYROIDISM; HPLC; ELISA;
D O I
10.1016/S0169-6009(08)80169-9
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Hydroxylysylpyridinoline (HP) and lysylpyridinoline (LP) are specific constituents of mature skeletal collagens excreted in urine. Their measurement represents a sensitive index of bone resorption. In this study, we have measured urinary excretion of pyridinolines crosslinks by immunoassay (ELISA) and HPLC methods in 80 patients with different bone resorption rates. We chose a sample of 44 healthy adults (30 men and 14 women) and a sample of 36 elderly patients (7 men and 29 women) presenting a secondary hyperparathyroidism due to a vitamin D deficiency. The correlation between HPLC (x) and ELISA (y) was judged satisfactory (y = 0.794x + 6.947, r = 0.92). The sensitivity of pyridinolines estimation was 50 nmol/l for immunoassay and 20 nmol/l for HPLC. The intra-assay and inter-assay coefficients of variation for the two analytical methods was <10%. The mean excretion of crosslinks (nmol/mmol of creatinine) measured by both methods in the sample of healthy adults was higher in women than in men. The amount of pyridinolines crosslinks excreted by elderly patients with vitamin D deficiency are three time higher than those of normal adults when measured by ELISA and HPLC methods. The distribution of different molecular forms of urinary pyridinoline crosslinks was investigated. Values of pyridinolines measured by HPLC in our samples of elderly patients have shown that free and peptide-bound pyridinolines with molecular weight (mel. wt.) smaller than 1000 Da represent approximately 80% of the total pyridinolines contained in urinary samples. A study on the evaluation of the antiserum used in the immunoassay for reacting with the different molecular forms isolated from urine showed a high affinity for free and peptide-bound pyridinolines with molecular weight smaller than 10 000 Da and that do not react strongly with peptide-bound with molecular weight greater than 10 000 Da. We conclude that, although this immunoassay does not measure total pyridinolines and does not distinguish between HP and LP, it seems convenient for diagnostic of metabolic bone diseases.
引用
收藏
页码:197 / 208
页数:12
相关论文
共 25 条
[1]   MONITORING OF FREE AND TOTAL URINARY PYRIDINOLINE AND DEOXYPYRIDINOLINE IN HEALTHY-VOLUNTEERS - SAMPLE RELATIONSHIPS BETWEEN 24-H AND FASTING EARLY MORNING URINE CONCENTRATIONS [J].
ABBIATI, G ;
BARTUCCI, F ;
LONGONI, A ;
FINCATO, G ;
GALIMBERTI, S ;
RIGOLDI, M ;
CASTIGLIONI, C .
BONE AND MINERAL, 1993, 21 (01) :9-19
[2]  
BEARDSWORTH LJ, 1990, J BONE MINER RES, V5, P671
[3]  
BETTICA P, 1992, CLIN CHEM, V38, P2313
[4]   URINARY PYRIDINIUM CROSS-LINKS AS MARKERS OF BONE-RESORPTION IN TUMOR-ASSOCIATED HYPERCALCEMIA [J].
BODY, JJ ;
DELMAS, PD .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1992, 74 (03) :471-475
[5]   CIRCULATING INTACT PARATHYROID-HORMONE MEASURED BY A 2-SITE IMMUNOCHEMILUMINOMETRIC ASSAY [J].
BROWN, RC ;
ASTON, JP ;
WEEKS, I ;
WOODHEAD, JS .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1987, 65 (03) :407-414
[6]  
DELMAS PD, 1993, J BONE MINER RES, V8, P643
[7]  
DELMAS PD, 1991, J BONE MINER RES, V6, P639
[8]  
EASTELL R, 1990, 3RD P INT S OST AALB, P469
[9]   SERUM CONCENTRATION OF THE CROSS-LINKED CARBOXYTERMINAL TELOPEPTIDE OF TYPE-I COLLAGEN (ICTP) IS A USEFUL PROGNOSTIC INDICATOR IN MULTIPLE-MYELOMA [J].
ELOMAA, I ;
VIRKKUNEN, P ;
RISTELI, L ;
RISTELI, J .
BRITISH JOURNAL OF CANCER, 1992, 66 (02) :337-341
[10]   COLLAGEN CROSS-LINKING IN HUMAN-BONE AND ARTICULAR-CARTILAGE - AGE-RELATED-CHANGES IN THE CONTENT OF MATURE HYDROXYPYRIDINIUM RESIDUES [J].
EYRE, DR ;
DICKSON, IR ;
VANNESS, K .
BIOCHEMICAL JOURNAL, 1988, 252 (02) :495-500