NUTRITIONAL REQUIREMENTS OF MOUSE ADRENAL CORTEX TUMOR CELLS IN CULTURE

被引:7
作者
CUPRAK, LJ
SATO, GH
机构
[1] Department of Dermatology, Tufts University School of Medicine, Boston
[2] Graduate Department of Biochemistry, Brandeis University, Waltham
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
D O I
10.1016/0014-4827(68)90503-X
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The minimal essential nutritional requirements for stable, highly differentiated clonal and non-clonal cultures of mouse adrenal cortex tumor cells were found to agree qualitatively with results reported earlier by Eagle for other mammalian cell lines which varied widely as to species and tissue origin. A requirement for tryptophan, biotin or vitamin B12 could not be positively established in the presence of low concentrations of dialyzed horse serum. It was not possible to demonstrate a loss in function without a parallel loss in total cellular protein under the experimental conditions cited. © 1968.
引用
收藏
页码:632 / &
相关论文
共 24 条
[1]  
BAILEY JL, 1962, TECHNIQUES PROTEINS
[2]   HORMONE-PRODUCING CULTURES OF ADRENAL AND PITUITARY TUMOR ORIGIN [J].
BUONASSISI, V ;
COHEN, AI ;
SATO, G .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1962, 48 (07) :1184-&
[3]  
COHEN AI, 1957, P SOC EXP BIOL MED, V95, P304
[5]  
EAGLE H, 1966, J BIOL CHEM, V241, P4994
[6]   POPULATION-DEPENDENT REQUIREMENT BY CULTURED MAMMALIAN CELLS FOR METABOLITES WHICH THEY CAN SYNTHESIZE [J].
EAGLE, H ;
PIEZ, K .
JOURNAL OF EXPERIMENTAL MEDICINE, 1962, 116 (01) :29-&
[7]   AMINO ACID METABOLISM IN MAMMALIAN CELL CULTURES [J].
EAGLE, H .
SCIENCE, 1959, 130 (3373) :432-437
[8]  
EAGLE H, 1955, T ASSOC AM PHYSICIAN, V68, P78
[9]  
EAGLE HARRY, 1955, JOUR BIOL CHEM, V214, P839
[10]  
EVANS VJ, 1956, CANCER RES, V16, P77