EFFECT OF SOME MONOHYDRIC ALCOHOLS ON THE FUNCTIONAL STABILITY OF BOVINE LIVER BETA-GALACTOSIDASE

被引：20

作者：

CORDONE, L

IZZO, V

SGROI, G

FORNILI, SL

机构：

[1] IST NAZL FIS NUCL, GRUPPO PALERMO, PALERMO, ITALY

[2] CNR, GRUPPI NAZL STRUCT MAT, PALERMO, ITALY

来源：

BIOPOLYMERS | 1979年 / 18卷 / 08期

关键词：

D O I：

10.1002/bip.1979.360180810

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The presence of some monohydric alcohols in the solution medium perturbs the kinetics of bovine liver .beta.-galactosidase. The effect increases with increasing alcohol concentration and alkyl group size. To explain this effect a model is suggested that considers the existence of functional and nonfunctional enzyme conformations in thermodynamical equilibrium. This equilibrium is dependent both on alcohol concentration and on alkyl group size.

引用

页码：1965 / 1974

页数：10

共 23 条

[1] Dielectric constants of some organic solvent-water mixtures at various temperatures
Akerlof, G
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1932, 54 : 4125 - 4139
[2] SOLVENT EFFECTS IN THE ALPHA-CHYMOTRYPSIN-HYDROCINNAMIC ESTER SYSTEM
BARNARD, ML
LAIDLER, KJ
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1952, 74 (23) : 6099 - 6101
[3] EFFECT OF APROTIC DIPOLAR ORGANIC SOLVENTS ON KINETICS OF ALPHA-CHYMOTRYPSIN-CATALYZED HYDROLYSES
CLEMENT, GE
BENDER, ML
[J]. BIOCHEMISTRY, 1963, 2 (04) : 836 - &
[4] EFFECT OF SOME MONOHYDRIC ALCOHOLS ON THE OXYGEN-AFFINITY OF HEMOGLOBIN - RELEVANCE OF SOLVENT DIELECTRIC-CONSTANT AND HYDROPHOBICITY
CORDONE, L
CUPANE, A
SANBIAGIO, PL
VITRANO, E
[J]. BIOPOLYMERS, 1979, 18 (08) : 1975 - 1988
[5] Eagland D., 1975, Water A Comprehensive Treatise: Aqueous Solutions of Amphiphiles and Macromolecules, P305, DOI [10.1007/978-1-4684-2958-9_5 LB - Eagland1975, DOI 10.1007/978-1-4684-2958-9_5LB-EAGLAND1975]
[6] Franks F., 1975, WATER COMPREHENSIVE, P1, DOI [10.1073/pnas.0610945104, DOI 10.1073/PNAS.0610945104]
[7] STUDIES OF CONFORMATION OF APOMYOGLOBIN IN AQUEOUS-SOLUTIONS AND DENATURING ORGANIC-SOLVENTS
HERSKOVITS, TT
SOLLI, NJ
[J]. BIOPOLYMERS, 1975, 14 (02) : 319 - 334
[8] HIPPEL PHV, 1965, J BIOL CHEM, V240, P3909
[9] KAUFMAN S, 1949, J BIOL CHEM, V180, P181
[10] MOLECULAR KINETICS OF MUSCLE ADENOSINE TRIPHOSPHATASE .2. SOLVENT AND STRUCTURAL EFFECTS
LAIDLER, KJ
ETHIER, MC
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1953, 44 (02) : 338 - 345

← 1 2 3 →