PREPARATION AND SEPARATION PROPERTIES OF OPEN TUBULAR COLUMNS WITH IMMOBILIZED CARBOWAX 20M FOR LC

被引：6

作者：

JANAK, K ^{[1
]}

HORKA, M ^{[1
]}

KREJCI, M ^{[1
]}

机构：

[1] CZECHOSLOVAK ACAD SCI,INST ANALYT CHEM,KOUNICOVA 42,CS-61142 BRNO,CZECHOSLOVAKIA

来源：

JOURNAL OF MICROCOLUMN SEPARATIONS | 1991年 / 3卷 / 02期

关键词：

MICRO LC; OPEN TUBULAR COLUMNS; IMMOBILIZED CARBOWAX 20M;

D O I：

10.1002/mcs.1220030204

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Open tubular columns (3-5 m x 23-50-mu-m i.d.) were coated with Carbowax 20M (0.07-0.2-mu-m film thickness) and immobilized with pluriisocyanates(Desmodur N or Desmodur L), a method which is simple, rapid, and reproducible. The concentration of cross-linking agent and the preparation conditions were optimized with respect to stability and separation properties of the immobilized film under liquid chromatography (LC) conditions. Properties of columns in LC systemsare strongly dependent on the degree of immobilization and slightly dependent on the type of Desmodur used as a cross-linking agent. The degree of immobilization must exceed 90% for successful application in LC. Such columns displayed good long-term stability, especially when immobilized with Desmodur L. Using water as the mobile phase, the LC system behaved as a reversed phase system. Capacity ratio and temperature strongly affected the separation efficiency.

引用

页码：115 / 120

页数：6

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