A QUANTITATIVE METHOD FOR SEPARATION OF LIVING HYDRA CELLS

被引：20

作者：

GREBER, MJ ^{[1
]}

DAVID, CN ^{[1
]}

HOLSTEIN, TW ^{[1
]}

机构：

[1] UNIV MUNICH, INST ZOOL, LUISENSTR 14, W-8000 MUNICH 2, GERMANY

来源：

ROUXS ARCHIVES OF DEVELOPMENTAL BIOLOGY | 1992年 / 201卷 / 05期

关键词：

CELL SEPARATION; COUNTERFLOW CENTRIFUGATION ELUTRIATION; CELL TYPE SPECIFIC GENE EXPRESSION; HYDRA;

D O I：

10.1007/BF00592110

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We describe a rapid method for the isolation of large numbers of living Hydra cells of defined cell type in an isotonic cell medium (Gierer et al. 1972). Intact animals are enzymatically dissociated into a single cell suspension and the various cell types separated in less than one hour by counterflow centrifugation elutriation. Cell loss is minimal. RNA isolated from various fractions can be probed with cell type specific cDNA-clones.

引用

页码：296 / 300

页数：5

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