THE CALMODULIN ANTAGONIST CALMIDAZOLIUM STIMULATES RELEASE OF NITRIC-OXIDE IN NEUROBLASTOMA N1E-115 CELLS

被引：12

作者：

HU, JR ^{[1
]}

ELFAKAHANY, EE ^{[1
]}

机构：

[1] UNIV MINNESOTA,SCH MED,DIV NEUROSCI RES PSYCHIAT,BOX 392,MAYO MEM BLDG,MINNEAPOLIS,MN 55455

来源：

NEUROREPORT | 1993年 / 4卷 / 02期

关键词：

NITRIC OXIDE; CALMODULIN ANTAGONISTS; CALMIDAZOLIUM; N1E-115; CELLS; CYCLIC GMP;

D O I：

10.1097/00001756-199302000-00021

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

EFFECTS of the calmodulin inhibitor calmidazolium on stimulation of nitric oxide (NO) release were investigated in neuroblastoma N1E-115 cells. NO release was determined indirectly by measuring cyclic GMP formation. Instead of the expected decrease in NO generation based on the calmodulin dependence of neuronal NO synthase, calmidazoline paradoxically increased cyclic GMP formation. Maximal activation occurred at 3 min and the effects were concentration dependent. This calmidazolium-stimulated NO release was markedly blocked by hemoglobin and N-monomethyl-L-arginine.

引用

页码：198 / 200

页数：3

共 10 条

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