SUBTOTAL BUT NOT UNILATERAL NEPHRECTOMY INDUCES HYPERPLASIA AND PROTOONCOGENE EXPRESSION

被引:23
作者
TERZI, F
TICOZZI, C
BURTIN, M
MOTEL, V
BEAUFILS, H
LAOUARI, D
ASSAEL, BM
KLEINKNECHT, C
机构
[1] HOP NECKER ENFANTS MALAD, INSERM, U192, F-75015 PARIS, FRANCE
[2] UNIV MILAN, CLIN PEDIAT MARCHI, I-20122 MILAN, ITALY
来源
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL FLUID AND ELECTROLYTE PHYSIOLOGY | 1995年 / 268卷 / 05期
关键词
SUBTOTAL NEPHRECTOMY; RENAL COMPENSATORY GROWTH; HYPERPLASIA;
D O I
10.1152/ajprenal.1995.268.5.F793
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
It is generally accepted that renal compensatory growth after unilateral nephrectomy (Uni) is due to prominent hypertrophy with no involvement of protooncogenes. Neither the balance between hypertrophy and hyperplasia nor the expression of the early-growth-related genes has been studied after subtotal nephrectomy (Nx). The occurrence of cystic tubular dilatations after Nx may suggest an excessive cell proliferation in this model. We measured DNA, RNA, and protein content, number of nuclei per tubular section, as well as c-fos, c-jun, c-myc, c-H-ras, c-sis, and c-erb-B2 protooncogene expression in kidneys taken at time of surgery and 2, 7, and 14 days after sham operation (control rats), Uni, or Nx. After Uni, hyperplasia was greater than expected (+79% for DNA at day 14) and was associated with moderate hypertrophy (+11% for protein/DNA ratio). After Nx, compensatory growth was due only to hyperplasia(+117% for DNA at day 14), with unchanged protein/DNA ratio (vs. Uni, P < 0.02). The greater hyperplasia after Nx was confirmed by nuclei counting. The protooncogene mRNA expression was constantly absent in control and Uni rats, whereas that of c-fos and c-jun genes was detected in Nx rats at day 14 with a 2- to 12-fold increment. The c-fos and c-jun protein levels were also increased at that time in Nx rats. This suggests the following: 1) the cellular events following Uni and Nx are not the same, and 2) the late protooncogene expression in Nx exclusively could favor a particular type of cell proliferation possibly more related with cystic formation than with actual compensatory growth.
引用
收藏
页码:F793 / F801
页数:9
相关论文
共 35 条
[1]   CONGENITAL MURINE POLYCYSTIC KIDNEY-DISEASE .2. PATHOGENESIS OF TUBULAR CYST FORMATION [J].
AVNER, ED ;
SWEENEY, WE ;
YOUNG, MC ;
ELLIS, D .
PEDIATRIC NEPHROLOGY, 1988, 2 (02) :210-218
[2]   SPECIFIC GENE-EXPRESSION DURING COMPENSATORY RENAL HYPERTROPHY IN THE RAT [J].
BEER, DG ;
ZWEIFEL, KA ;
SIMPSON, DP ;
PITOT, HC .
JOURNAL OF CELLULAR PHYSIOLOGY, 1987, 131 (01) :29-35
[4]   INFLUENCE OF AGE ON COMPENSATORY RENAL GROWTH IN RATS [J].
CELSI, G ;
JAKOBSSON, B ;
APERIA, A .
PEDIATRIC RESEARCH, 1986, 20 (04) :347-350
[5]   ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].
CHIRGWIN, JM ;
PRZYBYLA, AE ;
MACDONALD, RJ ;
RUTTER, WJ .
BIOCHEMISTRY, 1979, 18 (24) :5294-5299
[6]   ELEVATED C-MYC PROTOONCOGENE EXPRESSION IN AUTOSOMAL RECESSIVE POLYCYSTIC KIDNEY-DISEASE [J].
COWLEY, BD ;
SMARDO, FL ;
GRANTHAM, JJ ;
CALVET, JP .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (23) :8394-8398
[7]  
COWLEY BD, 1989, J BIOL CHEM, V264, P8389
[8]   THE BIOLOGY OF RENAL HYPERTROPHY [J].
FINE, LG .
KIDNEY INTERNATIONAL, 1986, 29 (03) :619-634
[9]   KINETICS OF COMPENSATORY GROWTH [J].
GOSS, RJ .
QUARTERLY REVIEW OF BIOLOGY, 1965, 40 (02) :123-&
[10]  
HALLIBURTON IW, 1965, CANCER RES, V25, P1882