SYNTHESIS AND ASSEMBLY OF DNA SUBUNITS IN ISOLATED HELA CELL NUCLEI

被引：75

作者：

KIDWELL, WR

MUELLER, GC

机构：

[1] McArdle Laboratory for Cancer Research University of Wisconsin, Madison

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1969年 / 36卷 / 05期

关键词：

D O I：

10.1016/0006-291X(69)90674-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nuclei from synchronized HeLa cells in S-phase continue to synthesize DNA in, vitro at or near the in, vivo replicating sites. Sedimentation of the product in an alkaline sucrose gradient reveals 10 and 24S segments in addition to the 40S non-replicating bulk DNA. Pulse chase experiments indicate that the 10S DNA is a precursor of the 24S product. ATP and the soluble protein fraction from the cytoplasm of S-phase cells support the accumulation of the 24S product. © 1969.

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页码：756 / &

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