SEQUENCE ORGANIZATION OF THE SOYBEAN GENOME

被引:82
作者
GURLEY, WB [1 ]
HEPBURN, AG [1 ]
KEY, JL [1 ]
机构
[1] UNIV GEORGIA, DEPT BOT, ATHENS, GA 30602 USA
关键词
(Soybean); DNA; Genome; Sequence interspersion;
D O I
10.1016/0005-2787(79)90500-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The total complexity of one constituent soybean (Glycine max) genome is estimated to be 1.29 · 109 nucleotide pairs, as determined by analysis of the reassociation kinetics of sheared (0.47 kilobase) DNA. Single copy sequences are estimated to represent from 53 to 64% of the genome by analysis of hydroxyapatite binding of repetitive DNA as a function of fragment length. From 65 to 70% of these sungle copy sequences have a short period interspetion with 1.11-1.36 kilobase lengths alternating with 0.3-0.4 kilobase repetitive sequence elements. The repetitive sequences of soybean DNA are interspersed both among themselves and among single copy regions of the genome. © 1979.
引用
收藏
页码:167 / 183
页数:17
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