MYRISTOYLATION OF FOOT-AND-MOUTH-DISEASE VIRUS CAPSID PROTEIN PRECURSORS IS INDEPENDENT OF OTHER VIRAL-PROTEINS AND OCCURS IN BOTH MAMMALIAN AND INSECT CELLS

被引：36

作者：

BELSHAM, GJ ^{[1
]}

ABRAMS, CC ^{[1
]}

KING, AMQ ^{[1
]}

ROOSIEN, J ^{[1
]}

VLAK, JM ^{[1
]}

机构：

[1] AGR UNIV WAGENINGEN, DEPT VIROL, 6700 EM WAGENINGEN, NETHERLANDS

来源：

JOURNAL OF GENERAL VIROLOGY | 1991年 / 72卷

关键词：

D O I：

10.1099/0022-1317-72-3-747

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The myristoylation of the foot-and-mouth disease virus (FMDV) capsid precursor P1-2A and its amino-terminal cleavage product 1AB, expressed from subgenomic cDNA, has been analysed. The modification reaction is independent of other FMDV proteins and occurs in both mammalian and insect cells. Blocking of the myristoylation site does not prevent efficient processing of the FMDV capsid precursor. A cDNA cassette in which the leader protease sequence is substituted by an ATG codon produces myristoylated 1AB, indicating correct removal of the novel N-terminal methionine residue.

引用

页码：747 / 751

页数：5

共 15 条

[1] INTRACELLULAR EXPRESSION AND PROCESSING OF FOOT-AND-MOUTH-DISEASE VIRUS CAPSID PRECURSORS USING VACCINIA VIRUS VECTORS - INFLUENCE OF THE L PROTEASE [J].

BELSHAM, GJ ;

BRANGWYN, JK ;

RYAN, MD ;

ABRAMS, CC ;

KING, AMQ .

VIROLOGY, 1990, 176 (02) :524-530

[2] A REGION OF THE 5' NONCODING REGION OF FOOT-AND-MOUTH-DISEASE VIRUS-RNA DIRECTS EFFICIENT INTERNAL INITIATION OF PROTEIN-SYNTHESIS WITHIN CELLS - INVOLVEMENT WITH THE ROLE OF L-PROTEASE IN TRANSLATIONAL CONTROL [J].

BELSHAM, GJ ;

BRANGWYN, JK .

JOURNAL OF VIROLOGY, 1990, 64 (11) :5389-5395

[3] FILM DETECTION METHOD FOR TRITIUM-LABELED PROTEINS AND NUCLEIC-ACIDS IN POLYACRYLAMIDE GELS [J].

BONNER, WM ;

LASKEY, RA .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1974, 46 (01) :83-88

[4] MYRISTYLATION OF PICORNAVIRUS CAPSID PROTEIN VP4 AND ITS STRUCTURAL SIGNIFICANCE [J].

CHOW, M ;

NEWMAN, JFE ;

FILMAN, D ;

HOGLE, JM ;

ROWLANDS, DJ ;

BROWN, F .

NATURE, 1987, 327 (6122) :482-486

[5] LEADER PROTEIN OF FOOT-AND-MOUTH-DISEASE VIRUS IS REQUIRED FOR CLEAVAGE OF THE P220 COMPONENT OF THE CAP-BINDING PROTEIN COMPLEX [J].

DEVANEY, MA ;

VAKHARIA, VN ;

LLOYD, RE ;

EHRENFELD, E ;

GRUBMAN, MJ .

JOURNAL OF VIROLOGY, 1988, 62 (11) :4407-4409

[6] NUCLEOTIDE-SEQUENCE AND GENOME ORGANIZATION OF FOOT-AND-MOUTH-DISEASE VIRUS [J].

FORSS, S ;

STREBEL, K ;

BECK, E ;

SCHALLER, H .

NUCLEIC ACIDS RESEARCH, 1984, 12 (16) :6587-6601

[7] EUKARYOTIC TRANSIENT-EXPRESSION SYSTEM BASED ON RECOMBINANT VACCINIA VIRUS THAT SYNTHESIZES BACTERIOPHAGE-T7 RNA-POLYMERASE [J].

FUERST, TR ;

NILES, EG ;

STUDIER, FW ;

MOSS, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (21) :8122-8126

[8]

KRAUSSLICH HG, 1990, J VIROL, V64, P2433

[9] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[10] LACK OF MYRISTOYLATION OF POLIOVIRUS CAPSID POLYPEPTIDE VP0 PREVENTS THE FORMATION OF VIRIONS OR RESULTS IN THE ASSEMBLY OF NONINFECTIOUS VIRUS-PARTICLES [J].

MARC, D ;

MASSON, G ;

GIRARD, M ;

VANDERWERF, S .

JOURNAL OF VIROLOGY, 1990, 64 (09) :4099-4107

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