EXPRESSION OF HUMAN RECOMBINANT CD23 IN INSECT CELLS

被引：14

作者：

JANSEN, KU ^{[1
]}

SHIELDS, J ^{[1
]}

GORDON, J ^{[1
]}

CAIRNS, J ^{[1
]}

GRABER, P ^{[1
]}

BONNEFOY, JY ^{[1
]}

机构：

[1] UNIV BIRMINGHAM,DEPT IMMUNOL,BIRMINGHAM B15 2TJ,W MIDLANDS,ENGLAND

来源：

JOURNAL OF RECEPTOR RESEARCH | 1991年 / 11卷 / 1-4期

关键词：

D O I：

10.3109/10799899109066424

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Human CD23 (low affinity receptor for IgE) has been expressed in insect cells (Sf9) using the baculovirus expression system and the baculovirus transfer vector pAc373. Insect cells infected with a recombinant baculovirus coding for CD23 synthesized a polypeptide not found in wild-type infected insect cells that had antigenic properties similar to natural CD23 produced in RPMI 8866 cells. Surface expression of recombinant CD23 was demonstrated by its ability to bind IgE. Recombinant CD23 expressed in insect cells had a slightly lower molecular weight (43 kDa) than that of natural CD23 (45 kDa) from RPMI 8866 cells as detected by SDS-PAGE followed by Western-blotting. Affinity-purified recombinant CD23 from infected insect cells showed B-cell growth promoting activity. These observations demonstrate for the first time that biologically active recombinant CD23 can be produced by the baculovirus expression system, thus providing a useful source of recombinant material to elucidate the biological functions of CD23.

引用

页码：507 / 520

页数：14

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