SOLID-PHASE EXTRACTION WITH SUPERCRITICAL FLUID ELUTION AS A SAMPLE PREPARATION TECHNIQUE FOR THE ULTRATRACE ANALYSIS OF FLAVONE IN BLOOD-PLASMA

被引:11
作者
LIU, HJ [1 ]
WEHMEYER, KR [1 ]
机构
[1] PROCTER & GAMBLE CO,MIAMI VALLEY LABS,POB 398707,CINCINNATI,OH 45239
来源
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 577卷 / 01期
关键词
D O I
10.1016/0378-4347(92)80598-K
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A new sample preparation technique. solid-phase extraction with supercritical fluid elution. was developed for the selective isolation of ultratrace levels of drugs from plasma. Plasma samples spiked with a drug were applied to octadecylsilane cartridges and the cartridges were then washed, briefly dried and directly fitted into cells for subsequent supercritical fluid elution. The absolute recovery was studied by using a radiolabeled model compound. The extraction selectivity was examined by chromatographing the extracts with a reversed-phase high-performance liquid chromatographic method with ultraviolet detection. The effects of extraction pressure and the length of capillary restrictors on drug recovery were examined in order to determine the optimal conditions for supercritical fluid elution. The performance of the method was compared to that of conventional solid-phase extraction in terms of recovery, selectivity, precision and accuracy of analysis. Flavone was used as the model compound and dog plasma as the biological matrix for these studies.
引用
收藏
页码:61 / 67
页数:7
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