DETECTION OF CUTANEOUS AND GENITAL HPV TYPES IN CLINICAL-SAMPLES BY PCR USING CONSENSUS PRIMERS

被引：161

作者：

TIEBEN, LM

TERSCHEGGET, J

MINNAAR, RP

BAVINCK, JNB

BERKHOUT, RJM

VERMEER, BJ

JEBBINK, MF

SMITS, HL

机构：

[1] UNIV AMSTERDAM, ACAD MED CTR,DEPT VIROL,FUNDAMENTAL VIROL SECT, MEIBERGDREEF 15, 1105 AZ AMSTERDAM, NETHERLANDS

[2] LEIDEN UNIV HOSP, DEPT DERMATOL, 2333 AA LEIDEN, NETHERLANDS

来源：

JOURNAL OF VIROLOGICAL METHODS | 1993年 / 42卷 / 2-3期

关键词：

HUMAN PAPILLOMAVIRUS; POLYMERASE CHAIN REACTION; CONSENSUS PRIMER;

D O I：

10.1016/0166-0934(93)90038-S

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two sets of consensus PCR primers consisting of a common 3' primer CP-I and two 5'-primers, CP-IIG (primer set A) and CP-IIS (primer set B), in the E1 open reading frame of the human papillomavirus (HPV) genome are presented. These two primer sets enabled the detection of a 188 base pair (bp) fragment of HPV 1, 2, 3, 4, 5, 6b, 7, 8, 9, 10a, 11, 12, 14a, 16, 17, 18, 19, 20, 21, 22, 24, 25, 31, 33, 36, 37, 38, 39 and 46. HPV types 15, 23, 49 and 50 were poorly amplified and HPV type 41 was not amplified. The method is suitable for the detection of HPV DNA sequences in clinical samples of both cervical and cutaneous lesions.

引用

页码：265 / 280

页数：16

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