CHARACTERIZATION OF FLUORINATED ETHYLCHLOROFORMATE DERIVATIVES OF PROTEIN AMINO-ACIDS USING POSITIVE AND NEGATIVE CHEMICAL-IONIZATION GAS-CHROMATOGRAPHY MASS-SPECTROMETRY

被引:19
作者
VATANKHAH, M [1 ]
MOINI, M [1 ]
机构
[1] UNIV TEXAS,DEPT CHEM & BIOCHEM,AUSTIN,TX 78712
关键词
D O I
10.1002/bms.1200230507
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
A solution of ethyl chloroformate + trifluoroethanol (or ethanol) + pyridine reacts rapidly with carboxylic and amino groups of amino acids to form n-ethoxycarbonyl amino acid trifluoroethyl (or ethyl) esters, which produce strong peaks representative of molecular ions in both positive and negative chemical ionization gas chromatography/mass spectrometry. Sensitivity of detection of the trifluoroethyl ester derivatives versus ethyl ester derivatives in the positive and negative chemical ionization modes is also discussed.
引用
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页码:277 / 282
页数:6
相关论文
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