A COLORIMETRIC ASSAY-METHOD FOR THE EVALUATION OF NEUROTROPHIC ACTIVITY IN-VITRO

被引：36

作者：

UEDA, Y

WALSH, E

NAKANISHI, H

YOSHIDA, K

机构：

[1] New Drug Research Laboratories, Fujisawa Pharmaceutical Co., Ltd. 2-1-6. Kashima, Yodogawa-ku, Osaka

来源：

NEUROSCIENCE LETTERS | 1994年 / 165卷 / 1-2期

关键词：

NEURONAL SURVIVAL; BIOASSAY; FIBROBLAST GROWTH FACTOR; COLORIMETRIC ASSAY; CORTEX; TISSUE CULTURE;

D O I：

10.1016/0304-3940(94)90745-5

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A colorimetric assay was established to detect neurotrophic activity by measuring the lysosomal enzyme, acid phosphatase (AP) activity of cultured neuronal cells. Neurons from the cerebral cortex of 14- or 15-day mouse embryo were cultured in serum-free medium for 3 days in 96-well culture plates. A linear relationship was obtained between the AP activity and the number of viable neurons counted under a microscope. The AP assay was used to evaluate the neurotrophic activity of basic fibroblast growth factor. This assay is shown to be simple, sensitive and convenient to detect neurotrophic activity.

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页码：203 / 207

页数：5

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