AN IMPROVED METHOD FOR ISOLATING RNA FROM COCCIDIAN OOCYSTS

被引：9

作者：

ABRAHAMSEN, MS

CLARK, TG

WHITE, MW

机构：

[1] Veterinary Molecular Biology, Montana State University, Bozeman

来源：

JOURNAL OF PARASITOLOGY | 1995年 / 81卷 / 01期

关键词：

D O I：

10.2307/3284017

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A method, based on the disruption of eimerian oocysts in a French pressure cell in the presence of guanidine isothiocyanate, has been developed to isolate large quantities of high quality total RNA efficiently. This procedure results in a 12.5-fold greater number of oocysts broken, and a 22-fold greater yield of total RNA than from disruption by conventional grinding in liquid N-2. In addition, the RNA isolated by the French pressure cell method was of equal or superior quality when compared to RNA isolated by grinding. This procedure provides a significant improvement in RNA extraction from eimerian oocysts and will greatly facilitate the study of gene expression in this important group of parasites.

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页码：107 / 109

页数：3

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