EXPRESSION OF A RICE GLUTELIN PROMOTER IN TRANSGENIC TOBACCO

被引：47

作者：

LEISY, DJ ^{[1
]}

HNILO, J ^{[1
]}

ZHAO, Y ^{[1
]}

OKITA, TW ^{[1
]}

机构：

[1] WASHINGTON STATE UNIV,INST BIOL CHEM,PULLMAN,WA 99164

来源：

PLANT MOLECULAR BIOLOGY | 1990年 / 14卷 / 01期

关键词：

dosage effect; glutelin; seed storage protein; tissue specificity; tobacco;

D O I：

10.1007/BF00015653

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A chimeric gene consisting of the 5′ flanking sequences of a rice glutelin gene (Gt3) linked to the chloramphenicol acetyltransferase (CAT) coding segment was introduced into tobacco via Agrobacterium tumefaciens-mediated transformation. CAT enzyme activity could be detected in extracts from seeds as early as 8 days after flowering and obtained a maximum level at 16 days after flowering, the onset of overall protein accumulation. Significant expression of CAT activity in non-seed tissues occurred in some, but not all plants, suggesting possible chromosome position effects on non-seed tissue expression. A positive correlation was observed between expression levels in seeds and gene copy numbers. © 1989 Kluwer Academic Publishers.

引用

页码：41 / 50

页数：10

共 26 条

[1] ACCELERATION OF NUCLEIC-ACID HYBRIDIZATION RATE BY POLYETHYLENE-GLYCOL [J].