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INHIBITION OF MUTANT TROPONIN-C ACTIVITY BY AN INTRA-DOMAIN DISULFIDE BOND

被引：111
作者：
GRABAREK, Z
TAN, RY
WANG, J
TAO, T
GERGELY, J
机构：
[1] HARVARD UNIV,SCH MED,DEPT NEUROL,BOSTON,MA 02115
[2] HARVARD UNIV,SCH MED,DEPT BIOL CHEM & MOLEC PHARMACOL,BOSTON,MA 02115
[3] MASSACHUSETTS GEN HOSP,NEUROL SERV,BOSTON,MA 02114
来源：
NATURE | 1990年 / 345卷 / 6271期
关键词：
D O I：
10.1038/345132a0
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Triggering of contraction in striated muscles involves a conformational transition in the N-terminal domain of troponin C, the calcium-binding component of thin filaments. We have designed a mutant troponin C in which the key conformational transition and the calcium-regulatory activity are reversibly blocked by the formation of a disulphide bridge. Our results may be applicable to other proteins of the same family of calcium-binding proteins. © 1990 Nature Publishing Group.
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页码：132 / 135
页数：4
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