CULTURED RAT OLFACTORY NEURONS ARE EXCITABLE AND RESPOND TO ODORS

被引：43

作者：

PIXLEY, SK ^{[1
]}

PUN, RYK ^{[1
]}

机构：

[1] UNIV CINCINNATI,COLL MED,DEPT PHYSIOL & BIOPHYS,CINCINNATI,OH 45267

来源：

DEVELOPMENTAL BRAIN RESEARCH | 1990年 / 53卷 / 01期

关键词：

Culture; Electrophysiology; Immunocytochemistry; Olfactory epithelium; Olfactory receptor neuron; Patch clamp; Rat;

D O I：

10.1016/0165-3806(90)90132-I

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Newborn rat nasal tissues containing olfactory epithelium were dissociated and maintained in a monolayer cell culture. Neurons were present, as determined by immunostaining with antibodies to 4 neuron-specific proteins: neuron-specific enolase, microtubule-associated protein 2, tau protein and synaptophysin. Immunostained neurons had a distinctive morphology resembling olfactory neurons. By patch-clamp analysis, these cells were electrically active. Responses of some neurons to physiological concentrations of an odorant mixture identified them as olfactory receptor cells. © 1990.

引用

页码：125 / 130

页数：6

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