SEVERAL MAMMALIAN UBIQUITIN CARRIER PROTEINS, BUT NOT E220K, ARE RELATED TO THE 20-KDA YEAST E2, RAD6

被引：11

作者：

BERLETH, ES

PICKART, CM

机构：

[1] Department of Biochemistry, SUNY at Buffalo

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 171卷 / 02期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0006-291X(90)91203-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Western blot analysis was used to probe the relationships between the multiple ubiquitin carrier proteins (E2s) of rabbit reticulocytes and the 20-kDa E2 encoded by the RAD6 gene of the yeast S. cerevisiae. Reticulocyte E2-14K, E2-17K, and E2-25K each reacted with two or more polyclonal anti-RAD6 antibody preparations; E2-20K, E2-35K, and E2-230K did not cross-react. These results suggest that some, but not all, reticulocyte E2s are members of a RAD6-like protein family which is conserved within and across species. RAD6 and E2-20K were also shown to multi-ubiquitinate histones by different mechanisms. © 1990.

引用

页码：705 / 710

页数：6

共 20 条

[1] A WATER-SOLUBLE, MONITORABLE PEPTIDE AND PROTEIN CROSS-LINKING AGENT [J].