GENETIC-MARKERS IN CULTURED PLANT-CELLS - DIFFERENTIAL SENSITIVITIES TO AMETHOPTERIN, AZETIDINE-2-CARBOXYLIC ACID AND HYDROXYUREA

被引:38
作者
NIELSEN, E [1 ]
ROLLO, F [1 ]
PARISI, B [1 ]
机构
[1] CNR,GENET BIOCHIM & EVOLUZIONIST LAB,I-27100 PAVIA,ITALY
来源
PLANT SCIENCE LETTERS | 1979年 / 15卷 / 02期
关键词
D O I
10.1016/0304-4211(79)90063-4
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The effects of three growth inhibitors, amethopterin, L-azetidine-2-carboxylic acid and hydroxyurea, have been tested on cell cultures from 6 plant species. The results show that naturally-occurring differences in drug sensitivity are present and can be easily demonstrated by assaying the effects of the drugs on the growth of cells in suspension culture under strictly-controlled experimental conditions. Similar differences are also evident in cells of the plants from which the cultures were derived. In order to determine the usefulness of these different sensitivities as selective tools, a culture flask was devised in which the effect of the drugs on a mixed culture of two cell species can be determined. © 1979.
引用
收藏
页码:113 / 125
页数:13
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