CHEMILUMINESCENCE - A SENSITIVE AND VERSATILE METHOD FOR ANALYTE DETECTION

被引：17

作者：

GIVENS, RS ^{[1
]}

JENCEN, DA ^{[1
]}

RILEY, CM ^{[1
]}

STOBAUGH, JF ^{[1
]}

CHOKSHI, H ^{[1
]}

HANAOKA, N ^{[1
]}

机构：

[1] UNIV KANSAS,CTR BIOANALYT RES,LAWRENCE,KS 66045

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1990年 / 8卷 / 06期

关键词：

Chemiluminescence; dioxetanedione; enkephalins; imidazole catalysis; mechanisms; activation energies; liquid chromatography; naphthalene-2,3-dialdehyde/cyanide; neurotensins; peroxyoxalate;

D O I：

10.1016/0731-7085(90)80057-V

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The generation of light from the oxidation of oxalic esters with hydrogen peroxide has been applied to the detection of luminescent materials. In order to improve the efficiency of this method, which is <0.1%, and to enhance the selectivity for target analytes, an in-depth investigation of the oxalate ester-hydrogen peroxide reaction has been conducted. A kinetic model has been developed based on the effects of catalysts, reagents and reaction conditions for maximum light production. Application of the model to liquid chromatography through the "time-dependent emission window" concept affords a predictable maximum sensitivity for selected analytes. Application to the detection and quantitation of met- and leu-enkephalins which have been labelled with naphthalene-2,3-dicarboxyaldehyde/cyanide provides support for this methodology. Other bioanalytical applications are presented. © 1990.

引用

页码：477 / 491

页数：15

共 28 条

[1] MULTIPLE INTERMEDIATES GENERATE FLUOROPHORE-DERIVED LIGHT IN THE OXALATE PEROXIDE CHEMILUMINESCENCE SYSTEM [J].