IDENTIFICATION OF PIG CHROMOSOMES IN PIG-MOUSE SOMATIC-CELL HYBRID BIVARIATE FLOW KARYOTYPES

被引：3

作者：

BOUVET, A ^{[1
]}

KONFORTOV, BA ^{[1
]}

MILLER, NGA ^{[1
]}

BROWN, D ^{[1
]}

TUCKER, EM ^{[1
]}

机构：

[1] AFRC,INST ANIM PHYSIOL & GENET RES,DEPT IMMUNOL,CAMBRIDGE RES STN,CAMBRIDGE CB2 4AT,ENGLAND

来源：

CYTOMETRY | 1993年 / 14卷 / 04期

关键词：

CELL LINES; CHROMOMYCIN-A3; CHROMOSOME MAPPING; CYTOGENETICS; DNA CONTENT; FLUORESCENCE; HOE-33258; HYBRIDOMAS; LYMPHOCYTES; SWINE;

D O I：

10.1002/cyto.990140405

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

To identify pig chromosomes in pig-mouse somatic cell hybrids, dual-laser flow karyotypes and GTG-banded metaphase spreads of pig, mouse, and 7 pig-mouse hybrid cell lines were compared. Pig chromosomes no. 1, 2, 5, 6, 10, 11, 13, 14, 16, 18, X and Y were tentatively assigned to individual peaks in the pig flow karyotype on the basis of DNA content vs. relative chromosome length. In the 7 hybrid cell lines, 7 out of 8 peaks distinct from those of the mouse cell line could be correlated with the presence of pig chromosomes no. 5,9, 10, 11 or 16,14,15, and 18, whereas 1 peak appeared to correspond to the presence of 1 middle-size chromosome (3, 4, or 7). Other pig chromosomes present in the hybrids could not be detected with certainty due to superposition with mouse peaks and mouse chromosome rearrangements.

引用

页码：369 / 376

页数：8

共 25 条

[1]

BLAISE F, 1990, ANN GENET-PARIS, V33, P146

[2] BIVARIATE FLOW KARYOTYPING OF HUMAN-CHROMOSOMES - EVALUATION OF VARIATION IN HOECHST-33258 FLUORESCENCE, CHROMOMYCIN-A3 FLUORESCENCE, AND RELATIVE CHROMOSOMAL DNA CONTENT [J].

BOSCHMAN, GA ;

RENS, W ;

VANOVEN, CH ;

MANDERS, EMM ;

ATEN, JA .

CYTOMETRY, 1991, 12 (06) :559-569

[3]

CHARDON P, 1991, ANIMAL GENETICS S1, V22, P86

[4] GENE-MAPPING BY ENZYMATIC AMPLIFICATION FROM FLOW-SORTED CHROMOSOMES [J].

COTTER, F ;

NASIPURI, S ;

LAM, G ;

YOUNG, BD .

GENOMICS, 1989, 5 (03) :470-474

[5]

DIXON SC, 1992, ANIM GENET, V23, P133

[6]

DIXON SC, 1990, 22 P INT C AN GEN E

[7]

FORD CE, 1980, HEREDITAS, V92, P145

[8] SWINE CHROMOSOMES - FLOW SORTING AND SPOT BLOT HYBRIDIZATION [J].

GRUNWALD, D ;

GEFFROTIN, C ;

CHARDON, P ;

FRELAT, G ;

VAIMAN, M .

CYTOMETRY, 1986, 7 (06) :582-588

[9]

GRUNWALD D, 1988, CYTOMETRIE FLUX ETUD, V2, P277

[10]

Gustavsson I, 1980, Adv Vet Sci Comp Med, V24, P245

← 1 2 3 →