INSECT RESISTANCE OF TRANSGENIC PLANTS THAT EXPRESS MODIFIED BACILLUS-THURINGIENSIS CRYIA(B) AND CRYIC GENES - A RESISTANCE MANAGEMENT STRATEGY

被引：157

作者：

VANDERSALM, T ^{[1
]}

BOSCH, D ^{[1
]}

HONEE, G ^{[1
]}

FENG, LX ^{[1
]}

MUNSTERMAN, E ^{[1
]}

BAKKER, P ^{[1
]}

STIEKEMA, WJ ^{[1
]}

VISSER, B ^{[1
]}

机构：

[1] DLO, CTR PLANT BREEDING & REPROD RES, DEPT MOLEC BIOL, 6700 AA WAGENINGEN, NETHERLANDS

来源：

PLANT MOLECULAR BIOLOGY | 1994年 / 26卷 / 01期

关键词：

BACILLUS THURINGIENSIS; CRYSTAL PROTEIN; PLANT; HELIOTHIS VIRESCENS; RESISTANCE MANAGEMENT; SPODOPTERA EXIGUA;

D O I：

10.1007/BF00039519

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Tobacco and tomato plants were generated exhibiting insect resistance due to the introduction of modified cryIA(b) and cryIC genes of Bacillus thuringiensis. Limited modifications at selected regions of the coding sequences of both genes are sufficient to obtain resistance against Spodoptera exigua, Heliothis virescens and Manduca sexta. The criteria used to modify both genes demonstrate that the removal of sequence motifs potentially resulting in premature polyadenylation and transcript instability causes increased insect resistance. The expression of a cryIC-cryIA(b) fusion resulting in protection against S. exigua, H. virescens and M. sexta demonstrates the potential of expressing translational fusions, not only to broaden the insect resistance of transgenic plants, but also to simultaneously employ different gene classes in resistance management strategies.

引用

页码：51 / 59

页数：9

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