MONOCLONAL-ANTIBODIES AGAINST METALLOPORPHYRINS - SPECIFICITY OF INTERACTION WITH STRUCTURALLY DIFFERENT METALLOPORPHYRINS

被引：18

作者：

SAVITSKY, AP

DEMCHEVA, MV

MANTROVA, EY

PONOMAREV, GV

机构：

[1] A. N. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, 117071

来源：

FEBS LETTERS | 1994年 / 355卷 / 03期

关键词：

MONOCLONAL ANTIBODY; METALLOPORPHYRIN; PD-COPROPORPHYRIN; HEMOPROTEIN; ABZYME;

D O I：

10.1016/0014-5793(94)01221-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Monoclonal antibodies against Pd-coproporphyrin I have been obtained. The antibody specificity for free as well as for conjugated Pd-coproporphyrin I is characterized. Affinity constants are estimated for 3 monoclonal antibodies effectively interacting with free Pd-coproporphyrin I. A comparative study on the binding of monoclonal antibodies with analogues and derivatives of Pd-coproporphyrin I has revealed that the antigen is mainly located inside the antibody paratope. The protein adjoins complementary to the metalloporphyrin in such a manner that antibodies obtained discern only isomer I, and to some degree, isomer III of coproporphyrin.

引用

页码：314 / 316

页数：3

共 12 条

[1] ANTIBODY-CATALYZED PORPHYRIN METALATION [J].