EFFECT OF DEAE-DEXTRAN AND DEXTRAN SULFATE ON [S-35]SULFATE INCORPORATION INTO GLYCOSAMINOGLYCANS OF NORMAL AND SV40 VIRUS-TRANSFORMED 3T3-CELLS

Short (10 min) and long (24 hr) treatments with 10 μg/ml dextran sulfate produced a marked increase in the incorporation of [35S] sulfate into cell-associated and extracellular glycosaminoglycans of SV40-transformed 3T3 cells. Similar treatment of 3T3 cells did not affect glycosaminoglycan synthesis. DEAE-dextran treatment for 24 hr produced a large increase in the cell-associated glycosaminoglycans and a proportional decrease in the extracellular sulfated glycosaminoglycans. The types of glycosaminoglycans synthesized were not affected significantly by the treatments. © 1979.