SENSITIVE FLUORESCENCE ASSAY FOR D,L-METHADONE

被引：1

作者：

CHI, CH ^{[1
]}

DIXIT, BN ^{[1
]}

机构：

[1] UNIV PITTSBURGH,SCH PHARM,DEPT PHARMACOL,PITTSBURGH,PA 15261

来源：

JOURNAL OF PHARMACEUTICAL SCIENCES | 1979年 / 68卷 / 09期

关键词：

Fluorometry—analysis; methadone in lung; liver; brain; serum; rats; Methadone—analysis; fluorescence assay; lung; Narcotic analgesics—methadone;

D O I：

10.1002/jps.2600680909

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

d,l‐Methadone forms a fluorophore when reacted with paraformaldehyde in concentrated sulfuric acid. Based on this reaction, a fluorescence assay suitable for quantitative d,l‐methadone analysis from plasma and other tissues was developed. d,l‐Methadone was extracted at pH 9.2 from the deproteinized filtrate of plasma or of aqueous tissue homogenate into an organic phase of 25% isobutanol in ethylene dichloride. After an aliquot of the organic phase was evaporated to dryness at 50‐55° with an air jet, paraformaldehyde (0.1%, in concentrated sulfuric acid) was added, and fluorescence was read at 450 nm with excitation at 275 nm. By this method, d,l‐ methadone can be assayed in the presence of its metabolites, morphine, diacetyimorphine (heroin), codeine, and cocaine; however, amphetamine, meperidine, and quinine interfere. Copyright © 1979 Wiley‐Liss, Inc., A Wiley Company

引用

页码：1097 / 1099

页数：3

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