NEGATIVE STAINING OF INTEGRAL MEMBRANE-PROTEINS

被引：7

作者：

BOEKEMA, EJ

机构：

[1] BIOSON Research Institute, University of Groningen, 9747 AG Groningen

来源：

MICRON AND MICROSCOPICA ACTA | 1991年 / 22卷 / 04期

关键词：

D O I：

10.1016/0739-6260(91)90054-4

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

This review describes aspects of negative staining of isolated integral membrane proteins. Detergents play a central role in the isolation of membrane proteins and also in their solubility in aqueous solutions. Specimens of mixed micelles of membrane proteins and non-ionic detergents can be easily prepared as long as the detergent concentration remains above the critical micellar concentration. Membrane proteins involved in the process of photosynthesis have been taken as examples to illustrate their interaction with different detergents. Upon negative staining, mixed micelles of membrane proteins and detergents show characteristic top and side view projections. On their sides, mixed micelles can easily aggregate into strings.

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页码：361 / 369

页数：9

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