IMMUNOLOGICAL IDENTIFICATION AND CHARACTERIZATION OF A DELAYED RECTIFIER K+ CHANNEL POLYPEPTIDE IN RAT-BRAIN

被引：238

作者：

TRIMMER, JS

机构：

[1] Department of Biochemistry, State University of New York, Stony Brook

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 23期

关键词：

EXCITABILITY; MAMMALIAN CENTRAL NERVOUS SYSTEM; ION-CHANNEL MOLECULAR BIOLOGY; TISSUE-SPECIFIC GENE EXPRESSION;

D O I：

10.1073/pnas.88.23.10764

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Antibodies specific for the drk1 polypeptide were used to characterize the corresponding protein in rat brain. Recombinant and synthetic immunogens containing fragments of the drk1 polypeptide were produced. Antibodies raised to these immunogens display monospecific reactions with the same 130-kDa polypeptide on immunoblots of adult rat brain membranes. Immunoprecipitation of I-125-labeled brain membranes identifies a 38-kDa peptide in tight association with the drk1 polypeptide. Immunohistochemical staining of sections of adult rat cortex shows that drk1 protein is restricted to neurons, where staining is present on dendrites and cell bodies but not on axons. These studies point to the value of such immunological reagents to the further characterization of the components of this delayed rectifier K+ channel in the mammalian central nervous system.

引用

页码：10764 / 10768

页数：5

共 25 条

[1] EXPRESSION OF A CLONED RAT-BRAIN POTASSIUM CHANNEL IN XENOPUS OOCYTES [J].