A SIMPLE TECHNIQUE FOR POSTEMBEDDING ULTRASTRUCTURAL IMMUNOGOLD LABELING

被引：9

作者：

LOCKARD, VG

机构：

[1] Department of Pathology, University of Mississippi Medical Center, Jackson, MS 39216

来源：

ULTRASTRUCTURAL PATHOLOGY | 1992年 / 16卷 / 1-2期

关键词：

IMMUNOGOLD LABELING; POSTEMBEDMENT IMMUNOLABELING; ULTRASTRUCTURAL IMMUNOLABELING;

D O I：

10.3109/01913129209074549

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

A simple and reproducible method for postembedding ultrastructural immunogold labeling is described. Tissues are fixed in Carson-Millonig phosphate-buffered 4% formaldehyde, and then are embedded in LR White resin. Immunogold labeling of ultrathin sections is accomplished by means of an indirect method that employs a nonconjugated primary antibody and gold particles conjugated to antibody to immunoglobulin G. Technical aspects of fixation, embedding, and immunolabeling that influence the success of this protocol are discussed.

引用

页码：47 / 50

页数：4

共 5 条

[1]

CARSON F, 1972, TEX REP BIOL MED, V30, P125

[2] THE PRESERVATION OF ULTRASTRUCTURE AND ANTIGENICITY [J].