APPLICATION OF THE HYDRA-ATTENUATA ASSAY FOR IDENTIFYING DEVELOPMENTAL HAZARDS AMONG NATURAL-WATERS AND WASTEWATERS

被引：10

作者：

FU, LJ ^{[1
]}

STAPLES, RE ^{[1
]}

STAHL, RG ^{[1
]}

机构：

[1] DUPONT CO,HASKELL LAB TOXICOL & IND MED,CENT RES & DEV,POB 50,ELKTON RD,NEWARK,DE 19714

来源：

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | 1991年 / 22卷 / 03期

关键词：

D O I：

10.1016/0147-6513(91)90081-Y

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

This study concerns application of the Hydra attenuata assay to detect the developmental toxicity potential of various aqueous samples. First, the assay was modified for testing aqueous samples because water quality has a major impact on aquatic toxicity testing and the results thus obtained. Ranges of sample pH, salinity (conductivity), and hardness were examined for their adverse effects upon the hydra. Adult hydra were unaffected morphologically by pH 5.5-9.5, and the artificial embryo ("embryo") developed normally in a pH range of 6.25 to 8.25. For water hardness, the minimal affective concentration was 1000 mg/liter (as CaCO3) in adults and 625 mg/liter in the embryos; the NOAELs for these were 750 mg/liter in the adult and 250 mg/liter CaCO3 in the embryo. Salinity in excess of 5 ppt was lethal to adults and embryos, indicating the assay may not be applicable to marine or highly saline samples. Finally, grab samples were tested from rivers in Maryland, Pennsylvania, New Jersey, and Delaware, some of which are impacted by industrial and agricultural activities, as well as several samples of industrial wastewaters from one major facility. The assay functioned normally with these diverse samples and yielded results that can be used in assessing the potential developmental hazard of these materials. © 1991.

引用

页码：309 / 319

页数：11

共 17 条

[1]

[Anonymous], 1984, GROUNDWATER CONTAMIN

[2]

Campbell R.D., 1983, P17

[3] TOXICOLOGY STUDIES OF A CHEMICAL-MIXTURE OF 25 GROUNDWATER CONTAMINANTS .3. MALE REPRODUCTION STUDY IN B6C3F1 MICE [J].

CHAPIN, RE ;

PHELPS, JL ;

SCHWETZ, BA ;

YANG, RSH .

FUNDAMENTAL AND APPLIED TOXICOLOGY, 1989, 13 (03) :388-398

[4]

DANSKY LA, COMMUNICATION

[5] PREDICTION OF THE DEVELOPMENTAL TOXICITY HAZARD POTENTIAL OF HALOGENATED DRINKING-WATER DISINFECTION BY-PRODUCTS TESTED BY THE INVITRO HYDRA ASSAY [J].

FU, LJ ;

JOHNSON, EM ;

NEWMAN, LM .

REGULATORY TOXICOLOGY AND PHARMACOLOGY, 1990, 11 (03) :213-219

[6] AN ARTIFICIAL EMBRYO FOR DETECTION OF ABNORMAL DEVELOPMENTAL BIOLOGY [J].

JOHNSON, EM ;

GABEL, BEG .

FUNDAMENTAL AND APPLIED TOXICOLOGY, 1983, 3 (04) :243-249

[7]

JOHNSON EM, 1984, ENVIRON HEALTH PERSP, V57, P135, DOI 10.2307/3429909

[8] SCREENING FOR TERATOGENIC HAZARDS - NATURE OF THE PROBLEMS [J].

JOHNSON, EM .

ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, 1981, 21 :417-429

[9]

JOHNSON EM, 1982, TERATOGEN CARCIN MUT, V2, P263, DOI 10.1002/1520-6866(1990)2:3/4<263::AID-TCM1770020308>3.0.CO

[10]

2-I

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