TRANSFORMATION AND INHERITANCE OF A HYGROMYCIN PHOSPHOTRANSFERASE GENE IN MAIZE PLANTS

被引：146

作者：

WALTERS, DA ^{[1
]}

VETSCH, CS ^{[1
]}

POTTS, DE ^{[1
]}

LUNDQUIST, RC ^{[1
]}

机构：

[1] PLANT SCI RES INC,10320 BREN RD E,MINNETONKA,MN 55343

来源：

PLANT MOLECULAR BIOLOGY | 1992年 / 18卷 / 02期

关键词：

HYGROMYCIN; INHERITANCE; MAIZE; TISSUE CULTURE; TRANSFORMATION;

D O I：

10.1007/BF00034948

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Embryogenic maize (Zea mays L.) callus cultures were transformed by microprojectile bombardment with a chimeric hygromycin phosphotransferase (HPT) gene and three transformed lines were obtained by selecting for hygromycin resistance. All lines contained one or a few copies of the intact HPT coding sequence. Fertile, transgenic plants were regenerated and the transmission of the chimeric gene was demonstrated through two complete generations. One line inherited the gene in the manner expected for a single, dominant locus, whereas two did not.

引用

页码：189 / 200

页数：12

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