Involvement of a common transcription factor in the regulated expression of IL-2 and IL-2 receptor genes

被引：63

作者：

Shibuya, H. ^{[1
]}

Yoneyama, M. ^{[1
]}

Taniguchi, T. ^{[1
]}

机构：

[1] Osaka Univ, Inst Mol & Cellular Biol, Suita, Osaka 565, Japan

来源：

INTERNATIONAL IMMUNOLOGY | 1989年 / 1卷 / 01期

关键词：

interleukins; regulatory DNA; regulatory transcription factor; cytokines; lymphotropic retrovirus;

D O I：

10.1093/intimm/1.1.43

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Interleukin-2 (IL-2) plays an essential role in the clonal expansion of antigen-activated T lymphocytes (T cells). In fact, the expression of both IL-2 and IL-2 receptor (IL-2R, p55, CD25) genes Is transiently Induced upon T cell activation through the Interaction of antigen/major histocompatibility complex (MHC) and T cell receptor complex. To elucidate the mechanism(s) of the induced gene expression for IL-2 and IL-2R, we have investigated for the presence of potential transcription factors that specifically interact with regulatory cis-elements. Here, we demonstrate that one such factor mediates the induced expression of both genes. Interestingly, the recognition sequences by this factor are significantly diverse in these two genes and are related to those of immunoglobulin (10) (kappa) chain and MHC class I genes. We provide evidence that this factor indeed binds to the IL-2, IL-2R, and Ig sequence elements with different affinities, thereby affecting the magnitude of gene expression. Interestingly, this factor also binds to other cytokine genes, such as interleukin-6 (IL-6), interferon-gamma (IFN-gamma), and HIV-1 and HTLV-1 LTR sequences.

引用

页码：43 / 49

页数：7

共 55 条

[1] MOLECULAR-CLONING OF A CD28 CDNA BY A HIGH-EFFICIENCY COS CELL EXPRESSION SYSTEM [J].

ARUFFO, A ;

SEED, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (23) :8573-8577

[2] ACTIVATION OF DNA-BINDING ACTIVITY IN AN APPARENTLY CYTOPLASMIC PRECURSOR OF THE NF-KAPPA-B TRANSCRIPTION FACTOR [J].

BAEUERLE, PA ;

BALTIMORE, D .

CELL, 1988, 53 (02) :211-217

[3] I-KAPPA-B - A SPECIFIC INHIBITOR OF THE NF-KAPPA-B TRANSCRIPTION FACTOR [J].

BAEUERLE, PA ;

BALTIMORE, D .

SCIENCE, 1988, 242 (4878) :540-546

[4] 2 TRANSCRIPTION FACTORS, NF-KAPPA-B AND H2TF1, INTERACT WITH A SINGLE REGULATORY SEQUENCE IN THE CLASS-I MAJOR HISTOCOMPATIBILITY COMPLEX PROMOTER [J].

BALDWIN, AS ;

SHARP, PA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (03) :723-727

[5] BINDING OF A NUCLEAR FACTOR TO A REGULATORY SEQUENCE IN THE PROMOTER OF THE MOUSE H-2KB CLASS-I MAJOR HISTOCOMPATIBILITY GENE [J].

BALDWIN, AS ;

SHARP, PA .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (01) :305-313

[6] STIMULATION OF OLIGODENDROGLIAL PROLIFERATION AND MATURATION BY INTERLEUKIN-2 [J].

BENVENISTE, EN ;

MERRILL, JE .

NATURE, 1986, 321 (6070) :610-613

[7]

Bohnlein E., 1988, CELL, V53, P827

[8] HUMAN CCAAT-BINDING PROTEINS HAVE HETEROLOGOUS SUBUNITS [J].

CHODOSH, LA ;

BALDWIN, AS ;

CARTHEW, RW ;

SHARP, PA .

CELL, 1988, 53 (01) :11-24

[9] REGULATION OF THE HUMAN INTERLEUKIN-2 RECEPTOR ALPHA-CHAIN PROMOTER - ACTIVATION OF A NONFUNCTIONAL PROMOTER BY THE TRANSACTIVATOR GENE OF HTLV-1 [J].

CROSS, SL ;

FEINBERG, MB ;

WOLF, JB ;

HOLBROOK, NJ ;

WONGSTAAL, F ;

LEONARD, WJ .

CELL, 1987, 49 (01) :47-56

[10]

Dignam J D., 1983, NUCLEIC ACIDS RES, V11, P1475

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