EXPRESSION OF CYTOCHROME-P-450 3A IN HT29-MTX CELLS AND CACO-2 CLONE TC7

被引：52

作者：

CARRIERE, V

LESUFFLEUR, T

BARBAT, A

ROUSSET, M

DUSSAULX, E

COSTET, P

DEWAZIERS, I

BEAUNE, P

ZWEIBAUM, A

机构：

[1] INSERM,U178,UNITE RECH DIFFERENCIAT CELLULAIRE INTESTINALE,F-94807 VILLEJUIF,FRANCE

[2] CHU NECKER ENFANTS MALAD,INSERM,U75,UNITE RECH BIOCHIM PHARMACOL & METAB,F-75730 PARIS 15,FRANCE

来源：

FEBS LETTERS | 1994年 / 355卷 / 03期

关键词：

CYTOCHROME P450 3A; CACO-2; CELL; HT-29;

D O I：

10.1016/0014-5793(94)01199-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

HT-29 sublines and Caco-2 clones were analyzed for the expression of cytochrome P-450 3A. The enzyme was found to be expressed in differentiated HT-29 cells selected by resistance to methotrexate and in one of seven Caco-2 clones, TC7. Its expression parallels the differentiation process, with highest levels being observed at late confluency. P-450 3A mRNA and protein patterns, as well as subcellular distribution, are intermediate between those observed in human adult intestine and fetal liver.

引用

页码：247 / 250

页数：4

共 35 条

[31] DIFFERENTIAL REGULATION OF CYTOCHROME-P-450 GENES ALONG RAT INTESTINAL CRYPT-VILLUS AXIS [J].