FORMATION OF A MINICHROMOSOME IN CRYPTOCOCCUS-NEOFORMANS AS A RESULT OF ELECTROPORATIVE TRANSFORMATION

被引：20

作者：

VARMA, A

KWONCHUNG, KJ

机构：

[1] Clinical Mycology Section, Laboratory-of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, 20892, MD

来源：

CURRENT GENETICS | 1994年 / 26卷 / 01期

关键词：

TRANSFORMATION; MINICHROMOSOME; YEAST; CRYPTOCOCCUS NEOFORMANS;

D O I：

10.1007/BF00326305

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A minichromosome of approximately 270 kilobases was generated following complementation of a ura5 mutant strain of C. neoformans with the plasmid pURA5g2. This is the first report of the in-vivo generation of a minichromosome by the method of electroporative transformation. The minichromosome occurred at a relatively high (>20%) frequency in transformants that were stable for uracil protoprophy. The minichromosome was maintained in linear form as a large extrachromosomal element of the normal karyotype. Gel-purified DNA from the minichromosome readily transformed the ura5 mutant of C. neoformans. Southern-blot analysis of the minichromosome revealed the presence of multiple copies of the URA5 gene and ribosomal DNA sequences in addition to containing telomere-like sequence rep eats. The minichromosome was transmitted through mitosis and meiosis with extremely-high fidelity.

引用

页码：54 / 61

页数：8

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