CULTURE OF CELLS FROM ZEBRAFISH (BRACHYDANIO-RERIO) BLASTULA-STAGE EMBRYOS

被引：26

作者：

GHOSH, C

COLLODI, P

机构：

[1] Department of Animal Sciences, Purdue University, West Lafayette, 47907, Indiana

来源：

CYTOTECHNOLOGY | 1994年 / 14卷 / 01期

关键词：

FISH CELL CULTURE; FISH EMBRYO; ZEBRAFISH;

D O I：

10.1007/BF00772192

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The zebrafish has become a popular model for studies of vertebrate development and toxicology. However, in vitro approaches utilizing this organism have not been fully exploited due to the absence of suitable cell culture systems. Previously, we developed methods for the culture of cells derived from zebrafish blastula-stage embryos. One of these cultures, ZEM-2, was derived in a complex medium containing trout embryo extract, trout serum and medium conditioned by buffalo rat liver cells. In this study we describe a zebrafish embryo cell line, ZEM-2A, derived from ZEM-2 following selection for growth in a simplified medium. Optimal growth of ZEM-2A cells is attained in nutrient medium supplemented with 5% fetal bovine serum.

引用

页码：21 / 26

页数：6

共 18 条

[1] CULTURE OF CELLS FROM ZEBRAFISH (BRACHYDANIO-RERIO) EMBRYO AND ADULT TISSUES [J].