SYNCHRONIZED BEATING OF EMBRYONIC MOUSE MYOCARDIAL CELLS MEDIATED BY FL CELLS IN MONOLAYER CULTURE

1. 1. A high percentage of single beating cells was obtained within one day in monolayer cultures of trypsin-dispersed embryonic mouse heart cells. 2. 2. The fastest single beating cells in younger cultures determined the beating rate of a monolayer sheet of cells in an older culture. 3. 3. For establishment of synchronous beating among myocardial cells direct contact between cells was not necessary. Synchronization of beating occurred even when two independently beating myocardial cells were connected through intermediate FL cells, strain cells derived from human amnion. © 1969.