A PROLINE TRANSPORT SYSTEM IN SACCHAROMYCES CHEVALIERI

被引:40
作者
MAGANASCHWENCKE, N
SCHWENCKE, J
机构
[1] Instituto de Química, Universidad de Chile, Valparaíso
关键词
D O I
10.1016/0005-2736(69)90114-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
1. 1. The properties of a proline accumulating system in nitrogen starved cells of Saccharomyces chevalieri are described. The system shows a Michaelis-Menten substrate dependence with an apparent Km of 2.5·10-5 M for l-proline and a Ki of 8.9·10-5 M for d-proline. 2. 2. The intracellular radioactivity accumulated corresponds to at least 85% free proline, as indicated by chromatography, displacement of accumulated proline and bio-assay. 3. 3. The uptake of proline is temperature dependent. Further, azide and 2.4-dinitrophenol inhibit the uptake. 4. 4. Measurement of the initial velocity of proline influx shows that the uptake of proline is not inhibited by a number of natural amino acids. Only α-alanine is inhibitory. l-Hydroxyproline and other amino acids are poor inhibitors. Some amino acids, such as tryptophan, phenylalanine and tyrosine, stimulate uptake. 5. 5. Among several structural analogs of proline, only sarcosine, l-thiazolidin-4-carboxylic acid, d- and l-azetidin carboxylic acid and 3,4-dehydro-dl-proline were found to be effective inhibitors of the system. All of these show a competitive type of inhibition. 6. 6. The results presented are consistent with the idea that the proline transport system in yeast is very specific. © 1969.
引用
收藏
页码:313 / +
页数:1
相关论文
共 38 条
[1]  
AHMED K, 1962, CAN J BIOCHEM PHYS, V40, P1101
[2]   ASSIMILATION OF ARGININE + LYSINE IN CANAVANINE RESISTANT + SENSITIVE STRAINS OF NEUROSPORA CRASSA [J].
BAUERLE, RH ;
GARNER, HR .
BIOCHIMICA ET BIOPHYSICA ACTA, 1964, 93 (02) :316-&
[3]  
BEGIN N, 1965, J BIOL CHEM, V240, P332
[4]   AMINO ACID ADSORPTION AND PROTEIN SYNTHESIS IN ESCHERICHIA COLI [J].
BRITTEN, RJ ;
ROBERTS, RB ;
FRENCH, EF .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1955, 41 (11) :863-870
[5]   EFFECTS OF AMINO ACIDS ON UTILIZATION OF TRYPTOPHAN + INDOLE FOR GROWTH BY MUTANT OF NEUROSPORA CRASSA [J].
BROCKMAN, HE .
JOURNAL OF GENERAL MICROBIOLOGY, 1964, 34 (01) :31-&
[6]  
CHRISTENSEN HN, 1960, MEMBRANE TRANSPORT M, P479
[7]   MECHANISM OF GLUCOSE TRANSPORT ACROSS YEAST CELL MEMBRANE [J].
CIRILLO, VP .
JOURNAL OF BACTERIOLOGY, 1962, 84 (03) :485-&
[8]   BACTERIAL PERMEASES [J].
COHEN, GN ;
MONOD, J .
BACTERIOLOGICAL REVIEWS, 1957, 21 (03) :169-194
[9]   Qualitative analysis of proteins: a partition chromatographic method using paper [J].
Consden, R ;
Gordon, AH ;
Martin, AJP .
BIOCHEMICAL JOURNAL, 1944, 38 :224-232
[10]   MOLECULAR TRANSPORT IN NEUROSPORA CRASSA .I. BIOCHEMICAL PROPERTIES OF A PHENYLALANINE PERMEASE [J].
DEBUSK, BG ;
DEBUSK, AG .
BIOCHIMICA ET BIOPHYSICA ACTA, 1965, 104 (01) :139-&