ALPHA-1-STIMULATED PHOSPHOINOSITIDE BREAKDOWN IN CULTURED CARDIOMYOCYTES - DIACYLGLYCEROL PRODUCTION AND COMPOSITION IN DOCOSAHEXAENOIC ACID SUPPLEMENTED CELLS

被引：39

作者：

BORDONI, A ^{[1
]}

BIAGI, PL ^{[1
]}

ROSSI, CA ^{[1
]}

HRELIA, S ^{[1
]}

机构：

[1] UNIV BOLOGNA,DIPARTMENTO BIOCHIM,VIA IRNERIO 48,I-40126 BOLOGNA,ITALY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 174卷 / 02期

关键词：

D O I：

10.1016/0006-291X(91)91498-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The fatty acid pattern of phosphatidylinositol and other inositol phospholipids is reported to be predominantly 1-stearoyl, 2-arachidonyl. However, literature does not report data about the effect of a modification of this fatty acid composition on the production and acidic pattern of the diacylglycerol (DAG) formed during phosphoinositide hydrolysis. Culturing cardiomyocytes in a docosahexaenoic acid supplemented medium, we obtained an homogeneous cell population whose phospholipid fatty acid pattern was strongly different from control cells, and which produced, after α1-adrenergic stimulation with phenylephrine, an higher amount of DAG. This DAG was different from control DAG in fatty acid composition, too. This structurally different DAG could be responsible for a different activation pattern of protein kinase C. © 1991.

引用

页码：869 / 877

页数：9

共 21 条

[1] INOSITOL TRISPHOSPHATE AND DIACYLGLYCEROL AS 2ND MESSENGERS [J].