MOLECULAR CHARACTERIZATION OF A NONAUTONOMOUS TRANSPOSABLE ELEMENT (DTPH1) OF PETUNIA

被引:90
作者
GERATS, AGM
HUITS, H
VRIJLANDT, E
MARANA, C
SOUER, E
BELD, M
机构
[1] FREE UNIV AMSTERDAM,DEPT GENET,1081 HV AMSTERDAM,NETHERLANDS
[2] VRIJE UNIV BRUSSELS,VIROL LAB,B-1640 RHODE ST GENESE,BELGIUM
关键词
D O I
10.1105/tpc.2.11.1121
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
An insertion sequence of 283 base pairs has been isolated from the DFR-C gene (dihydroflavonol-4-reductase) of petunia. This insert was found only in a line unstable for the An1 locus (anthocyanin 1, located on chromosome VI) and not in fully pigmented progenitor and revenant lines or in stable white derivative lines. This implies that the An1 locus encodes the DFR-C gene. The unstable An1 system in the line W138 is known to be a two-element system, the autonomous element being located on chromosome I. In the presence of the autonomous element, W138 flowers exhibit a characteristic pattern of red revertant spots and sectors on a white background. In the absence of the autonomous element, the W138 allele gives rise to a stable recessive (white) phenotype. Sequence analysis of progenitor, unstable, and revenant alleles revealed dTph1 to contain perfect terminal inverted repeats of 12 base pairs. In DFR-C, it is flanked by an 8-base pair target site duplication. Sequences homologous to dTph1 are present in at least 50 copies in the line W138. Sequence analysis of An1 revertant alleles indicated that excision, including removal of the target site duplication, is required for reversion to the wild-type phenotype. Derivative stable recessive alleles showed excision of dTph1 and a rearrangement of the target site duplication. dTph1 is the smallest transposable element described to date that is still capable of transposition. The use of dTph1 in tagging experiments and subsequent gene isolation is discussed.
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页码:1121 / 1128
页数:8
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