DETECTION OF TRANSMEMBRANE LINKAGES BETWEEN IMMUNOGLOBULIN OR COMPLEMENT RECEPTORS AND THE NEUTROPHILS CORTICAL MICROFILAMENTS BY RESONANCE ENERGY-TRANSFER MICROSCOPY

被引：25

作者：

ZHOU, MJ ^{[1
]}

TODD, RF ^{[1
]}

PETTY, HR ^{[1
]}

机构：

[1] UNIV MICHIGAN,SCH MED,DEPT INTERNAL MED,ANN ARBOR,MI 48109

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1991年 / 218卷 / 02期

关键词：

D O I：

10.1016/0022-2836(91)90709-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

By exploiting the 1 r4 (where r is the separation distance between fluorochromes) dependence of energy transfer between parallel lamellae we have observed transmembrane energy transfer between membrane receptors and cortical microfilaments. Receptors were labeled with donor- or acceptor-conjugated Fab fragments whereas microfilaments were labeled with acceptor- or donor-conjugated phalloidin reagents. Energy transfer was imaged by optical microscopy.We report that cell surface receptors can be constitutively unlinked, linked or inducibly linked to microfilaments. © 1991.

引用

页码：263 / 268

页数：6

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