DEVELOPMENT OF AN ELISA FOR THE DETECTION OF 2-NITROIMIDAZOLE HYPOXIA MARKERS BOUND TO TUMOR-TISSUE

被引：18

作者：

RALEIGH, JA ^{[1
]}

ZEMAN, EM ^{[1
]}

RATHMAN, M ^{[1
]}

LADINE, JK ^{[1
]}

CLINE, JM ^{[1
]}

THRALL, DE ^{[1
]}

机构：

[1] N CAROLINA STATE UNIV,COLL VET MED,DEPT ANAT PHYSIOL SCI & RADIOL,RALEIGH,NC 27606

来源：

INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS | 1992年 / 22卷 / 03期

关键词：

TUMOR HYPOXIA; HYPOXIA MARKERS; 2-NITROIMIDAZOLES; CCI-103F; ELISA; CANINE TUMORS;

D O I：

10.1016/0360-3016(92)90841-5

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Canine and rodent tumors covalently bind the fluorinated 2-nitroimidazole, CCI-103F, in a way that immunohistochemical analysis shows is consistent with the location of tumor hypoxia. We have now developed a rapid, quantitative, and non-radioactive enzyme linked immunosorbent assay for the binding of CCI-103F in biopsy samples of spontaneous canine tumors. Issues of antigen stability during tissue processing, calibration of the ELISA, and the use of biopsy samples for measuring tumor hypoxia by the ELISA approach are addressed.

引用

页码：403 / 405

页数：3

共 7 条

[1] IMMUNOHISTOCHEMICAL DETECTION OF A HYPOXIA MARKER IN SPONTANEOUS CANINE TUMORS [J].

CLINE, JM ;

THRALL, DE ;

PAGE, RL ;

FRANKO, AJ ;

RALEIGH, JA .

BRITISH JOURNAL OF CANCER, 1990, 62 (06) :925-931

[2] DYNAMIC MEASUREMENTS OF HEXAFLUOROMISONIDAZOLE (CCI-103F) RETENTION IN MOUSE-TUMORS BY H-1 F-19 MAGNETIC-RESONANCE SPECTROSCOPY [J].

JIN, GY ;

LI, SJ ;

MOULDER, JE ;

RALEIGH, JA .

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1990, 58 (06) :1025-1034

[3] DEMONSTRATION OF TUMOR-SELECTIVE RETENTION OF FLUORINATED NITROIMIDAZOLE PROBES BY F-19 MAGNETIC-RESONANCE SPECTROSCOPY INVIVO [J].

MAXWELL, RJ ;

WORKMAN, P ;

GRIFFITHS, JR .

INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 1989, 16 (04) :925-929

[4] COVALENT BINDING OF A FLUORINATED 2-NITROIMIDAZOLE TO EMT-6 TUMORS IN BALB/C MICE - DETECTION BY F-19 NUCLEAR-MAGNETIC-RESONANCE AT 2.35-T [J].

RALEIGH, JA ;

FRANKO, AJ ;

TREIBER, EO ;

LUNT, JA ;

ALLEN, PS .

INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 1986, 12 (07) :1243-1245

[5] FLUORESCENCE IMMUNOHISTOCHEMICAL DETECTION OF HYPOXIC CELLS IN SPHEROIDS AND TUMORS [J].

RALEIGH, JA ;

MILLER, GG ;

FRANKO, AJ ;

KOCH, CJ ;

FUCIARELLI, AF ;

KELLY, DA .

BRITISH JOURNAL OF CANCER, 1987, 56 (04) :395-400

[6]

RALEIGH JA, 1991, IN PRESS MAGN RES ME

[7] BINDING OF H-3 MISONIDAZOLE TO SOLID HUMAN-TUMORS AS A MEASURE OF TUMOR HYPOXIA [J].

URTASUN, RC ;

CHAPMAN, JD ;

RALEIGH, JA ;

FRANKO, AJ ;

KOCH, CJ .

INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 1986, 12 (07) :1263-1267

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