ULTRASTRUCTURAL ORGANIZATION OF TYPE-XI COLLAGEN IN FETAL BOVINE EPIPHYSEAL CARTILAGE

被引:33
作者
PETIT, B
RONZIERE, MC
HARTMANN, DJ
HERBAGE, D
机构
[1] INST BIOL & CHIM PROTEINES,CNRS,UPR 412,F-69367 LYON 7,FRANCE
[2] INST PASTEUR,CTR RADIOANAL,CNRS,URA 1459,F-69365 LYON 7,FRANCE
关键词
D O I
10.1007/BF00269096
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Type XI collagen was localized with polyclonal antibodies specific for alpha1(XI) and alpha2(XI) chains in the resting zone of epiphyseal cartilage from calf fetuses. The immunofluorescence technique was used on sections of cartilage, and the immunogold labelling technique for electron microscopy on fibrils isolated from cartilage and, for the fist time, in situ on blocks of cartilage fractured in liquid nitrogen. Immunofluorescence showed that without pepsin treatment the staining of type XI collagen was restricted to the pericellular zones; after pepsin treatment, the staining was co-distributed with that of type II collagen. Immunoelectron microscopy performed on isolated fibrils and on cartilage blocks showed that after disruption of fibrils with pepsin, type XI collagen was labelled on small filaments on the fibrils. When the fibrils were not disrupted, labelling was observed in situ only at the ends of the fibrils or on cross-sections of some fibrils. These results indicate that type XI collagen is located inside type II collagen fibrils in fetal bovine epiphyseal cartilage, as already postulated for embryonic chicken sterna.
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收藏
页码:231 / 239
页数:9
相关论文
共 33 条
[1]  
BERNARD M, 1988, J BIOL CHEM, V263, P17159
[2]   COLLAGEN TYPE-I AND TYPE-V ARE PRESENT IN THE SAME FIBRIL IN THE AVIAN CORNEAL STROMA [J].
BIRK, DE ;
FITCH, JM ;
BABIARZ, JP ;
LINSENMAYER, TF .
JOURNAL OF CELL BIOLOGY, 1988, 106 (03) :999-1008
[3]  
BRUCKNER P, 1988, J BIOL CHEM, V263, P16911
[4]  
DASILVA PP, 1984, IMMUNOLABELLING ELEC, P179
[5]  
EIKENBERRY EF, 1992, ARTICULAR CARTILAGE, P132
[6]   LOCALIZATION OF COLLAGEN TYPES AND FIBRONECTIN IN CARTILAGE BY IMMUNOFLUORESCENCE [J].
EVANS, HB ;
AYAD, S ;
ABEDIN, MZ ;
HOPKINS, S ;
MORGAN, K ;
WALTON, KW ;
WEISS, JB ;
HOLT, PJL .
ANNALS OF THE RHEUMATIC DISEASES, 1983, 42 (05) :575-581
[7]   COLLAGEN TYPE-IX - EVIDENCE FOR COVALENT LINKAGES TO TYPE-II COLLAGEN IN CARTILAGE [J].
EYRE, DR ;
APON, S ;
WU, JJ ;
ERICSSON, LH ;
WALSH, KA .
FEBS LETTERS, 1987, 220 (02) :337-341
[8]  
EYRE DR, 1987, J RHEUMATOL, V14, P25
[9]  
EYRE DR, 1991, J RHEUMATOL, V18, P49
[10]   DIFFERENT LEVELS OF GLYCOSYLATION CONTRIBUTE TO THE HETEROGENEITY OF ALPHA-1(II) COLLAGEN CHAINS DERIVED FROM A TRANSPLANTABLE RAT CHONDROSARCOMA [J].
FURUTO, DK ;
MILLER, EJ .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1983, 226 (02) :604-611