FLOW-INJECTION DETERMINATION OF GLUCOSE IN SERUM WITH AN IMMOBILIZED PYRANOSE OXIDASE REACTOR

被引：12

作者：

KIBA, N ^{[1
]}

UEDA, F ^{[1
]}

FURUSAWA, M ^{[1
]}

YAMANE, T ^{[1
]}

机构：

[1] YAMANASHI UNIV,FAC EDUC,DEPT CHEM,KOFU,YAMANASHI 400,JAPAN

来源：

ANALYTICA CHIMICA ACTA | 1992年 / 269卷 / 02期

关键词：

CHEMILUMINESCENCE; ENZYMATIC METHODS; FLOW INJECTION; GLUCOSE; SERUM;

D O I：

10.1016/0003-2670(92)85401-Q

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A flow-injection system for the chemiluminometric determination Of D-glucose in serum with an immobilized pyranose oxidase reactor is described. Pyranose oxidase is immobilized on controlled-pore glass beads and packed into a stainless-steel column. Serum (100 nl) is diluted 1000-fold with water. Sample solution (10 mul) is injected into the carrier stream. The hydrogen peroxide produced is detected by measuring the chemiluminescence emitted on admixing with luminol and potassium hexacyanoferrate(III). The maximum sample throughput is 60 h-1. The calibration graph is linear from 0.2 to 500 muM glucose; the detection limit is 0.05 muM. The immobilized enzyme reactor is stable for at least 2 months.

引用

页码：187 / 191

页数：5

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