HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF INSULIN SYNTHESIS IN BIOLOGICAL-SYSTEMS

被引：1

作者：

CALVARUSO, G ^{[1
]}

TESORIERE, G ^{[1
]}

VENTO, R ^{[1
]}

GIULIANO, M ^{[1
]}

CARABILLO, M ^{[1
]}

机构：

[1] CTR ONCOBIOL SPERIMENTALE,PALERMO,ITALY

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 660卷 / 02期

关键词：

D O I：

10.1016/0378-4347(94)00303-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper reports a two-step high-performance liquid chromatographic procedure which permits the study of the incorporation of [H-3]leucine into insulin in biological systems. The first step of the procedure was size exclusion chromatography, performed on a GPC-100 column, which was eluted with 0.1 M KH2PO4-methanol (9:1, v/v). By this step the bulk of both protein and radioactivity was separated from tritiated insulin. The second step, which employs reversed-phase chromatography on an octadecylsilyl column, permits the separation of insulin from other contaminants by means of a linear gradient of acetonitrile. This simple and reproducible method was employed to test insulin synthesis in cultured human retinoblastoma Y79 cells.

引用

页码：259 / 264

页数：6

共 14 条

[1] INSULIN IN THE BRAIN [J].