DELAYED TRANSLATION AND POSTTRANSLATIONAL PROCESSING OF CYRITESTIN, AN INTEGRAL TRANSMEMBRANE PROTEIN OF THE MOUSE ACROSOME

被引：61

作者：

LINDER, B ^{[1
]}

BAMMER, S ^{[1
]}

HEINLEIN, UAO ^{[1
]}

机构：

[1] UNIV DUSSELDORF,INST GENET,D-40225 DUSSELDORF,GERMANY

来源：

EXPERIMENTAL CELL RESEARCH | 1995年 / 221卷 / 01期

关键词：

D O I：

10.1006/excr.1995.1353

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

This paper presents data on the cellular localization of the testis-expressed mouse Cyrn gene product, cyritestin. This cysteine-rich protein is a member of a family including various rodent and primate proteins and snake venom proteins of the metalloproteinase and disintegrin types. By using antibodies raised against recombinant proteins generated in bacteria and against synthetic peptides we show that (i) Cyrn mRNA is present in germ cells 4 days prior to translation; (ii) cyritestin protein is localized in the acrosomal region of spermatids and spermatozoa; and (iii) cyritestin has an apparent molecular weight of 110,000 Daltons, but is subject to processing during epididymal sperm transport, resulting in a shorter molecule lacking approximately 55 kDa from the N-terminal half. We conclude that cyritestin becomes exposed on the sperm surface after successful acrosome reaction and thus may play a role in sperm function rather than in testicular germ cell maturation. (C) 1995 Academic Press, Inc.

引用

页码：66 / 72

页数：7

共 45 条

[1] A COMMON PRECURSOR FOR A PUTATIVE HEMORRHAGIC PROTEIN AND RHODOSTOMIN, A PLATELET-AGGREGATION INHIBITOR OF THE VENOM OF CALLOSELASMA-RHODOSTOMA - MOLECULAR-CLONING AND SEQUENCE-ANALYSIS [J].